nucleation period meaning in Chinese

成核阶段
成核期

The his - tagged peacl - gfp purified from the supernatants could polymerize into green fluorescent filamentous structures with diameter , length and shape being identical to that of muscle f - actins , which could be labeled by tritc - phalloidin ( a specific agent for staining actin microfilaments ) , and were identified as having a 9 nm diameter by negative staining , corresponding with that of the muscle f - actins ( 7 - 10 nm ) . under polymerization conditions , his - tagged peacl - gfp polymerized with kinetics similar to those of skeleton muscle actin , that is , an obvious lag nucleation period at the beginning of polymerization and an s - like typical polymerization curve could be obtained . the critical concentration is 0 . 75 umol / l near to that of chicken muscle actin ( 0 . 56 umol / l ) under the same condition
荧光标记结合荧光显微观察表明：从可溶性上清中纯化的his - taggedpeac1 - gfp聚合形成的微丝不仅可以直接在荧光显微镜下观察，也可被微丝的特异标记物鬼笔环肽所标记，而且其直径、长度以及形态上与已知的聚合肌动蛋白荧光丝一致；电镜负染的结果进一步证实其直径为9nm ，与传统微丝直径相当( 7 ? 10nm ) ；聚合曲线有明显的停滞期，为典型的s型聚合曲线，聚合临界浓度为0 . 75 mol l ，这一结果与已有报道相似。